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human breast cancer cell line mcf 7  (ATCC)


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    Structured Review

    ATCC human breast cancer cell line mcf 7
    Human Breast Cancer Cell Line Mcf 7, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 34713 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/breast+cancer+cell+lines+mcf+7/MCF7/pmc13101642-243-7-13
    Average 99 stars, based on 34713 article reviews
    human breast cancer cell line mcf 7 - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Cell Culture:

    Article Title: The adipogenesis-related gene CEBPD as a prognostic biomarker and functional tumor suppressor in breast cancer.
    Article Snippet: .. Cell culture The normal breast epithelial cell line HBL-100 and the breast cancer cell lines MCF-7, BT-474, and HCC1806 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). .. All cell lines were cultured in Dulbecco's Modified Eagle's Medium (DMEM; Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS; Gibco) and 1% penicillin/streptomycin (Gibco).

    Article Title: Cold atmospheric plasma restores fulvestrant sensitivity by inhibiting CCND3 in the drug-resistant breast cancer cells
    Article Snippet: .. Breast cancer cell lines MCF-7 and T47D were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA) and cultured in RPMI 1640 medium (Welgene, Gyeongsan, Korea) supplemented with 10% fetal bovine serum (Gibco BRL, Carlsbad, CA, USA) and 2% penicillin/streptomycin (Capricorn Scientific, Ebsdorfergrund, Germany) under humidified 5% CO2 at 37 °C. .. To generate fulR cells, MCF-7 and T47D cells were continuously exposed to increasing doses of fulvestrant (Selleck Chemicals, Houston, TX, USA) for 32 weeks, up to 4.0 and 3.0 nM, respectively.

    Article Title: miR-3648 regulates cell functions and acts as a potential biomarker to predict prognosis in breast cancer and its bioinformatics analysis.
    Article Snippet: .. AR TIC LE IN PR ES S ARTICLE IN PRESS Cell line selection and cultivation The breast cancer cell lines MCF-7 (RRID:CVCL_0031) and BT-474 (RRID:CVCL_0179), alongside the normal mammary epithelial line MCF-10A (RRID:CVCL_0598) (all sourced from ATCC), were cultured under standard conditions (37°C, 5% CO2). ..

    Article Title: Pachyphylinin A-B: two novel seco-apotirucallane triterpenoids with cytotoxic activities from the stem bark of Aglaia pachyphylla.
    Article Snippet: Extended author information available on the last page of the article Abstract New seco-apotirucallane triterpenoids, pachyphylinin A (1) and B (2), were isolated from the stem bark of Aglaia pachyphylla alongside nine triterpenoid compounds (3–11).. The structures were elucidated and identified through various spectroscopic analyses, including 1D and 2D NMR, HRTOF-MS, FTIR, as well as ECD, complemented by computational calculation using TD-DFT.. In the process, pachyphylinin A (1) and B (2) were tested for cytotoxic activity against the breast cancer cell line (MCF-7).

    Multiple Displacement Amplification:

    Article Title: miR-214 Promotes Aggressive Behavior in Triple-Negative Breast Cancer by Functionally Targeting the 3′-UTR of FRK
    Article Snippet: .. The human breast epithelial cell line MCF-10A (ATCC ® CRL-10317TM) and breast cancer cell lines MCF-7 (ATCC ® HTB-22TM), MDA-MB-231 (ATCC ® HTB-26TM), and MDA-MB-468 (ATCC ® HTB-132TM) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). ..

    Article Title: Chemical profiling and biological activities of <i>Athamanta sicula</i> L. Aerial parts extracts: <i>in vitro</i> and in <i>silico</i> approaches
    Article Snippet: .. Breast cancer cell lines MCF-7 and MDA-MB-231 (ATCC, Germany) were maintained as described in our earlier work[4]. .. They were cultured in DMEM medium (Biological Industries) supplemented with 10% fetal bovine serum, 1% L-glutamine, and 1% penicillin-streptomycin, and incubated at 37°C under 95% humidity with 5% CO2.

    Article Title: A phenothiazine analog, PTF, suppresses breast cancer growth by inhibiting YAP signaling.
    Article Snippet: Cycloheximide, MG132, chloroquine (CQ), 3-methyladenine, and staurosporine were purchased from Sigma-Aldrich (St. Louis, MO, USA). .. The breast cancer cell lines MCF-7, MDA-MB-231, and Hs578T were originally established and distributed by the American Type Culture Collection (Manassas, VA, USA). .. These cells were expanded in DMEM/ F12 medium (Invitrogen, Carlsbad, CA, USA) formulated with 10 % fetal bovine serum (Gibco, Grand Island, NY, USA).

    Selection:

    Article Title: miR-3648 regulates cell functions and acts as a potential biomarker to predict prognosis in breast cancer and its bioinformatics analysis.
    Article Snippet: .. AR TIC LE IN PR ES S ARTICLE IN PRESS Cell line selection and cultivation The breast cancer cell lines MCF-7 (RRID:CVCL_0031) and BT-474 (RRID:CVCL_0179), alongside the normal mammary epithelial line MCF-10A (RRID:CVCL_0598) (all sourced from ATCC), were cultured under standard conditions (37°C, 5% CO2). ..

    Sequencing:

    Article Title: HIF-2α expression is controlled by the progesterone receptor and regulates hCG-induced gene expression in granulosa cells during ovulation in mice.
    Article Snippet: Ovulation is induced via a surge in gonadotrophin hormones, which increases the expression of the essential ovulatory transcription factor progesterone receptor (PGR) and its target genes.. The importance of PGR in ovulation is well defined; however, the role of its many downstream genes largely remains unknown.. Using mouse models of ovulation, we show that the Epas1 gene, which encodes the hypoxia inducible transcription factor 2α (HIF-2α), is expressed in a PGR-dependent manner during ovulation.

    Expressing:

    Article Title: HIF-2α expression is controlled by the progesterone receptor and regulates hCG-induced gene expression in granulosa cells during ovulation in mice.
    Article Snippet: Ovulation is induced via a surge in gonadotrophin hormones, which increases the expression of the essential ovulatory transcription factor progesterone receptor (PGR) and its target genes.. The importance of PGR in ovulation is well defined; however, the role of its many downstream genes largely remains unknown.. Using mouse models of ovulation, we show that the Epas1 gene, which encodes the hypoxia inducible transcription factor 2α (HIF-2α), is expressed in a PGR-dependent manner during ovulation.

    Control:

    Article Title: HIF-2α expression is controlled by the progesterone receptor and regulates hCG-induced gene expression in granulosa cells during ovulation in mice.
    Article Snippet: Ovulation is induced via a surge in gonadotrophin hormones, which increases the expression of the essential ovulatory transcription factor progesterone receptor (PGR) and its target genes.. The importance of PGR in ovulation is well defined; however, the role of its many downstream genes largely remains unknown.. Using mouse models of ovulation, we show that the Epas1 gene, which encodes the hypoxia inducible transcription factor 2α (HIF-2α), is expressed in a PGR-dependent manner during ovulation.



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    Analysis of the selected senescence markers and anillin levels in HCT116 p53WT <t>and</t> <t>MCF-7</t> cells induced to senesce by 1 day-treatment with doxorubicin and collected 5 days after senescence induction. ( A, B ) Densitometric analysis of protein levels in HCT116 p53WT ( A ) and MCF-7 ( B ), n=9; statistical analysis was performed using paired one-tailed t-Student test. Relative protein expression means fold change (in the expression of proteins relative to the expression of GAPDH) vs appropriate control. Boxes: Q1, median, Q3; error bars: Minimum, Maximum. ( C ) Representative images from Western blotting of HCT116 p53WT and MCF-7 cell lysates. ( D, E ) Analysis of fluorescence intensity of anillin (whole nucleus area) and representative images ( F, G ) of control and doxorubicin-treated HCT116 p53WT ( D, F ) and MCF-7 cells ( E, G ), n=3; statistical analysis was performed using Wilcoxon matched-pairs signed-rank test. Data on graphs represent individual values for analyzed cells, median, error bars: Minimum, Maximum. Red – anillin, blue – DAPI stained DNA. Scale 20 µm. Statistical significance relative to control: ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001
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    Image Search Results


    Cytotoxic effect of compounds 5a-5e on MCF-7 cells following exposure to different concentrations (10, 20, 60, and 100 μM). Data are presented as mean ± SD, n = 3. *P < 0.05, **P < 0.01, and *** P < 0.001 indicate significant differences in comparison with the negative control group (untreated cells); doxorubicin was used as the positive control.

    Journal: Research in Pharmaceutical Sciences

    Article Title: Molecular docking, synthesis, and cytotoxic evaluation of novel quinazoline-quinazolinone hybrid compounds

    doi: 10.4103/RPS.RPS_150_24

    Figure Lengend Snippet: Cytotoxic effect of compounds 5a-5e on MCF-7 cells following exposure to different concentrations (10, 20, 60, and 100 μM). Data are presented as mean ± SD, n = 3. *P < 0.05, **P < 0.01, and *** P < 0.001 indicate significant differences in comparison with the negative control group (untreated cells); doxorubicin was used as the positive control.

    Article Snippet: The MCF-7 (human breast cancer) cell line was acquired from the Pasteur Institute of Iran (Tehran, Iran).

    Techniques: Comparison, Negative Control, Positive Control

    Analysis of the selected senescence markers and anillin levels in HCT116 p53WT and MCF-7 cells induced to senesce by 1 day-treatment with doxorubicin and collected 5 days after senescence induction. ( A, B ) Densitometric analysis of protein levels in HCT116 p53WT ( A ) and MCF-7 ( B ), n=9; statistical analysis was performed using paired one-tailed t-Student test. Relative protein expression means fold change (in the expression of proteins relative to the expression of GAPDH) vs appropriate control. Boxes: Q1, median, Q3; error bars: Minimum, Maximum. ( C ) Representative images from Western blotting of HCT116 p53WT and MCF-7 cell lysates. ( D, E ) Analysis of fluorescence intensity of anillin (whole nucleus area) and representative images ( F, G ) of control and doxorubicin-treated HCT116 p53WT ( D, F ) and MCF-7 cells ( E, G ), n=3; statistical analysis was performed using Wilcoxon matched-pairs signed-rank test. Data on graphs represent individual values for analyzed cells, median, error bars: Minimum, Maximum. Red – anillin, blue – DAPI stained DNA. Scale 20 µm. Statistical significance relative to control: ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001

    Journal: Aging and Disease

    Article Title: Anillin Recedes in p53-Dependent Senescence of Tumor Cells and Reappears in Cells Escaping from Senescence

    doi: 10.14336/AD.2025.0402

    Figure Lengend Snippet: Analysis of the selected senescence markers and anillin levels in HCT116 p53WT and MCF-7 cells induced to senesce by 1 day-treatment with doxorubicin and collected 5 days after senescence induction. ( A, B ) Densitometric analysis of protein levels in HCT116 p53WT ( A ) and MCF-7 ( B ), n=9; statistical analysis was performed using paired one-tailed t-Student test. Relative protein expression means fold change (in the expression of proteins relative to the expression of GAPDH) vs appropriate control. Boxes: Q1, median, Q3; error bars: Minimum, Maximum. ( C ) Representative images from Western blotting of HCT116 p53WT and MCF-7 cell lysates. ( D, E ) Analysis of fluorescence intensity of anillin (whole nucleus area) and representative images ( F, G ) of control and doxorubicin-treated HCT116 p53WT ( D, F ) and MCF-7 cells ( E, G ), n=3; statistical analysis was performed using Wilcoxon matched-pairs signed-rank test. Data on graphs represent individual values for analyzed cells, median, error bars: Minimum, Maximum. Red – anillin, blue – DAPI stained DNA. Scale 20 µm. Statistical significance relative to control: ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001

    Article Snippet: The human HCT116 p53-proficient (referred to as HCT116 p53WT) colon cancer cell line and the breast cancer cell line MCF-7 were obtained from ATCC (HCT116 CCL-247; MCF-7 HTB-22).

    Techniques: One-tailed Test, Expressing, Control, Western Blot, Fluorescence, Staining

    Correlation between p53 and anillin levels during senescence and the escape from senescence in breast cancer MCF-7 and colon cancer HCT116 p53WT cells (see ). ( A-B ). Representative Western blots showing the levels of anillin and p53 in HCT116 p53WT cells ( A ) and MCF-7 cells ( B ). ( C-F ) The level of anillin and p53 on subsequent days of cell culture after senescence induction by doxorubicin in HCT116 p53WT ( C and E ) and MCF-7 cells ( D and F ) n = 4; statistical analysis was performed using one-way ANOVA followed by post hoc analysis (Tukey’s honest significant difference test; HSD test). Statistical significance of differences between indicated days of treatment: * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001. Boxes: Q1, median, Q3; error bars: Minimum, Maximum. ( G ) The heat map shows the levels of anillin and p53 during senescence and escape from senescence in MCF-7 and HCT116 p53WT cells. Heatmap: Original data points are standardized into z-scores.

    Journal: Aging and Disease

    Article Title: Anillin Recedes in p53-Dependent Senescence of Tumor Cells and Reappears in Cells Escaping from Senescence

    doi: 10.14336/AD.2025.0402

    Figure Lengend Snippet: Correlation between p53 and anillin levels during senescence and the escape from senescence in breast cancer MCF-7 and colon cancer HCT116 p53WT cells (see ). ( A-B ). Representative Western blots showing the levels of anillin and p53 in HCT116 p53WT cells ( A ) and MCF-7 cells ( B ). ( C-F ) The level of anillin and p53 on subsequent days of cell culture after senescence induction by doxorubicin in HCT116 p53WT ( C and E ) and MCF-7 cells ( D and F ) n = 4; statistical analysis was performed using one-way ANOVA followed by post hoc analysis (Tukey’s honest significant difference test; HSD test). Statistical significance of differences between indicated days of treatment: * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001. Boxes: Q1, median, Q3; error bars: Minimum, Maximum. ( G ) The heat map shows the levels of anillin and p53 during senescence and escape from senescence in MCF-7 and HCT116 p53WT cells. Heatmap: Original data points are standardized into z-scores.

    Article Snippet: The human HCT116 p53-proficient (referred to as HCT116 p53WT) colon cancer cell line and the breast cancer cell line MCF-7 were obtained from ATCC (HCT116 CCL-247; MCF-7 HTB-22).

    Techniques: Western Blot, Cell Culture